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Antibodies
Anti-DCAMKL1 antibody
Anti-DCAMKL1 antibody from Abcam
Be the first to write a review!
Citations:
(111)
Supplier Page
Supplier Page from
Abcam for
Anti-DCAMKL1 antibody
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
Anti-DCAMKL1 antibody
Company
Abcam
Price
Pricing Info
Supplier Page
View Supplier Product Page
Catalog Number
ab31704
Antibody Type
Primary
Isotype
IgG
Clonality
Polyclonal
Applications
Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western blot
Host
Rabbit
Quantity
100 UG
Conjugate/Tag
Unconjugated
Concentration
0.5 mg/ml
Gene Name
DCLK1
Validation
KnockOut Validated
Reactivity
Human, Mouse, Rat
Target
DCAMKL1
Add to Compare List
Abcam
Discovery Drive
Cambridge Biomedical Campus
Cambridge
CB2 0AX
United Kingdom
Phone
: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600
Company Profile
Website:
http://www.abcam.com
Citations (111)
(1)
Generation, culture, and stimulation of small intestinal murine organoids in parasitology research
STAR Protocols
December 1, 2023
Marta Campillo Poveda, Claire Drurey, Rick M. Maizels
REAGENT or RESOURCE,SOURCE,IDENTIFIER Antibodies,Antibodies,Antibodies ,,
Anti-DCAMKL1
antibody,
Abcam
,
ab31704
"Goat anti-rabbit IgG (H + L) secondary antibody, FITC",
Invitrogen
,
65-6111
Fixable Viability Dye eFluor
(2)
Partial in vivo reprogramming enables injury-free intestinal regeneration via autonomous Ptgs1 induction
Science Advances
November 24, 2023
Jumee Kim, Somi Kim, Seung-Yeon Lee, Beom-Ki Jo, Ji-Young Oh, Eun-Ji Kwon, Keun-Tae Kim, Anish Ashok Adpaikar, Eun-Jung Kim, Han-Sung Jung,
et al.
1:100
), rabbit anti–E-cadherin (
Cell Signaling Technology
,
3195
,
1:1000
), and rabbit anti-Dclk1(
Abcam
,
ab31704
,
1:1000
).
Histology
and AB-PAS
staining
For histological analysis, sections were stained with hematoxylin
(3)
Wnt activation disturbs cell competition and causes diffuse invasion of transformed cells through NF-κB-MMP21 pathway
Nature Communications
November 3, 2023
Kazuki Nakai, Hancheng Lin, Shotaro Yamano, Shinya Tanaka, Sho Kitamoto, Hitoshi Saitoh, Kenta Sakuma, Junpei Kurauchi, Eilma Akter, Masamitsu Konno,
et al.
were used in this study: chicken anti-GFP (
ab13970
), rabbit anti-Ki-67 (
ab16667
), rabbit anti-DCLK1 (
ab31704
), rabbit anti-LYVE1 (
ab14917
), mouse anti-chromogranin A (
ab80787
) and rabbit anti-p65 (
ab16502
) antibodies
(4)
Tuft cells utilize taste signaling molecules to respond to the pathobiont microbe Ruminococcus gnavus in the proximal colon
Frontiers in Immunology
October 25, 2023
Hao Lei, Defu Yu, Yan-Bo Xue, Yi-Hong Li, Shi-Meng Gong, Yuan-Yuan Peng, Kai-Fang Liu, Damiano Buratto, Yisen Yang, Sai-Sai Zhang,
et al.
were applied at room temperature for 1 h. Primary antibodies used were as follows: anti-
Dclk1
(
Abcam
,
ab31704
), Alexa Fluor® 488 Rabbit monoclonal to
DCAMKL1
(
Abcam
,
ab202754
), anti-PLCß2 (
Santa Cruz Biotechnology
,
(5)
Sterile triggers drive joint inflammation in TNF‐ and IL‐1β‐dependent mouse arthritis models
EMBO Molecular Medicine
October 11, 2023
Alexandra Thiran, Ioanna Petta, Gillian Blancke, Marie Thorp, Guillaume Planckaert, Maude Jans, Vanessa Andries, Korneel Barbry, Elisabeth Gilis, Julie Coudenys,
et al.
with primary antibody (rabbit anti‐lysozyme (Dako; EC 3.2.1.17, 1/500) or rabbit
anti‐DCAMKL1
(
Abcam
;
Ab31704
, 1/500)) overnight at 4°C. After washing the slides with PBS, ileum sections were counterstained with
(6)
Single-Cell RNA Sequencing (scRNA-seq) Identifies L1CAM as a Key Mediator between Epithelial Tuft Cell and Innate Lymphoid Cell in the Colon of Hnrnp I Knockout Mice
Biomedicines
October 9, 2023
Guanying (Bianca) Xu, Yuan-Xiang Pan, Wenyan Mei, Hong Chen
the respective
procedures
. In brief, primary antibodies of anti-DCAMKL1/DCLK1 (Abcam, Cambridge, UK,
ab31704
, dilution at
1:200
), anti-L1CAM (
Abcam
, Cambridge, UK,
ab24345
, dilution at
1:100
), anti-
GATA3
(
Abcam
,
(7)
IFN-λ derived from nonsusceptible enterocytes acts on tuft cells to limit persistent norovirus
Science Advances
September 15, 2023
Harshad Ingle, Heyde Makimaa, Somya Aggarwal, Hongju Deng, Lynne Foster, Yuhao Li, Elizabeth A. Kennedy, Stefan T. Peterson, Craig B. Wilen, Sanghyun Lee,
et al.
hybridizations with MNoV probes (Opal 520; Akoya Biosciences) in combination with anti-DCLK1 antibody (
Abcam
;
ab31704
) on fixed frozen or paraffin-embedded sections. Tissues were stained with anti-DCLK1 antibody overnight
(8)
Proteomic investigation of neural stem cell to oligodendrocyte precursor cell differentiation reveals phosphorylation-dependent Dclk1 processing
Cellular and molecular life sciences : CMLS
August 18, 2023
Hardt R, Dehghani A, Schoor C, Gödderz M, Cengiz Winter N, Ahmadi S, Sharma R, Schork K, Eisenacher M, Gieselmann V,
et al
.
blocking solution: anti-myc-tag (
1:5000
, rabbit pAb,
ab9106
,
Abcam
, UK), anti-Dclk1 (
1:1000
, rabbit pAb,
ab31704
,
Abcam
, UK),
anti-Padi2
(
1:1000
, rabbit pAb,
12110-1-AP
,
Proteintech Group
, Rosemont, IL), anti-Pura
(9)
Diet-mediated constitutive induction of novel IL-4+ ILC2 cells maintains intestinal homeostasis in mice
Journal of Experimental Medicine
August 7, 2023
Wanlin Cui, Yuji Nagano, Satoru Morita, Takeshi Tanoue, Hidehiro Yamane, Keiko Ishikawa, Toshiro Sato, Masato Kubo, Shohei Hori, Tadatsugu Taniguchi,
et al.
(
103134
;
BioLegend
),
EPCAM
(
118214
;
BioLegend
) and DCLK1 (primary antibody, rabbit anti-DCLK1,
Abcam
ab31704
; secondary antibody, goat anti-rabbit IgG-AF488,
Life Technologies
). Tuft cells were defined as the
(10)
An organoid-based CRISPR-Cas9 screen for regulators of intestinal epithelial maturation and cell fate
Science advances
July 14, 2023
Hansen SL, Larsen HL, Pikkupeura LM, Maciag G, Guiu J, Müller I, Clement DL, Mueller C, Johansen JV, Helin K,
et al
.
610154
; RRID: AB_397555), and anti-DCLK1 (
1:400
dilution; polyclonal rabbit antibody;
Abcam
, catalog no.
ab31704
; RRID: AB_873537). Each well was washed three times for 5 min with PBS, before
incubation
for 1 to 2
(11)
Combined multidimensional single-cell protein and RNA profiling dissects the cellular and functional heterogeneity of thymic epithelial cells
Nature communications
July 10, 2023
Klein F, Veiga-Villauriz C, Börsch A, Maio S, Palmer S, Dhalla F, Handel AE, Zuklys S, Calvo-Asensio I, Musette L,
et al
.
BD Biosciences
), CD146-APC (
1:800
, ME-9F1, Biolegend), EpCMA1-PerCPCy5.5 (
1:800
, G8.8, Biolegend), Dclk1 (
1:1000
,
ab31704
,
Abcam
), HVEM-APC (
1:400
, HMHV-1B18, Biolegend), Ly51-PECy7 (
1:400
, 6C3, Biolegend), MHCII-APC/Fire750
(12)
Targeting DCLK1 attenuates tumor stemness and evokes antitumor immunity in triple-negative breast cancer by inhibiting IL-6/STAT3 signaling
Breast cancer research : BCR
April 17, 2023
Liu H, Yan R, Xiao Z, Huang X, Yao J, Liu J, An G, Ge Y.
USA) using chemiluminescence kit (Millipore, USA). The primary antibodies used above were: anti-DCLK1(
ab31704
,
Abcam
), anti-
SOX2
(
3579T
,
CST
), anti-
NANOG
(
4903T
,
CST
), anti-
OCT4
(
11263-1-AP
,
Proteintech
), anti-
CD44
(13)
Embryonic keratin19+ progenitors generate multiple functionally distinct progeny to maintain epithelial diversity in the adult thymus medulla
Nature Communications
April 12, 2023
Beth Lucas, Andrea J. White, Fabian Klein, Clara Veiga-Villauriz, Adam Handel, Andrea Bacon, Emilie J. Cosway, Kieran D. James, Sonia M. Parnell, Izumi Ohigashi,
et al.
2312434), K19 (EP1580Y,
Abcam
, Cat no:
ab52625
, Lot no: GR3384962-1), DCLK1 (DCAMKL1,
Abcam
, Cat No:
ab31704
, Lot no: GR3357375-3),
CCL21
(
LifeSpan
Technologies, Cat no:
LS-C104634
, Lot no: 55059), SSEA-1-BV421
(14)
CDK8 and CDK19 act redundantly to control the CFTR pathway in the intestinal epithelium
EMBO reports
February 6, 2023
Susana Prieto, Geronimo Dubra, Alain Camasses, Ana Bella Aznar, Christina Begon‐Pescia, Elisabeth Simboeck, Nelly Pirot, François Gerbe, Lucie Angevin, Philippe Jay,
et al.
C,Polyclonal,gift,Rabbit,
PCNA
,Monoclonal,
Santa Cruz
,
Mouse
,
sc‐56
Lysozyme,Polyclonal,Dako,Rabbit,A0099 Anti‐Dclk1,Polyclonal,
Abcam
,Rabbit,
Ab31704
Olfm4,Monoclonal,
Cell signaling
,Rabbit,
39141
β‐Catenin,Monoclonal,BD BioSciences,
Mouse
,"610,154" BrdU,Monoclonal,DSHB,
Mouse
,G3G4 RNA
(15)
Loss of SLC26A3 Results in Colonic Mucosal Immune Dysregulation via Epithelial-Immune Cell Crosstalk
Cellular and molecular gastroenterology and hepatology
January 1, 2023
Jayawardena D, Priyamvada S, Kageyama T, White Z, Kumar A, Griggs TF, Majumder A, Akram R, Anbazhagan AN, Sano T,
et al
.
"
ROR gamma (t) Monoclonal Antibody (B2D), PE, eBioscience
"
Novus
,
NBP1-75516
,
IL-33
Invitrogen
,
PA5-47007
,
IL-33
Millipore
,
05-1567
,P-120 Catenin
Abcam
,
ab31704
,DCLK1 Research Resource Center UIC and Pocono Rabbit Farm,Research Resource Center UIC and Pocono Rabbit
(16)
Defining the early stages of intestinal colonisation by whipworms
Nature Communications
December 1, 2022
María A. Duque-Correa et al.
α-Ki-67 (
1:250
,
Abcam
,
ab16667
), α-chromogranin A (
1:50
,
Abcam
,
ab15160
), α-Dcamlk-1 (
1:200
,
Abcam
,
ab31704
), α-zona occludens-1 (
ZO-1
) protein (
1:200
,
Invitrogen
,
61-7300
) and the lectins
Ulex europaeus
agglutinin
(17)
Sirt6-mediated epigenetic modification of DNA accessibility is essential for Pou2f3-induced thymic tuft cell development
Communications Biology
December 1, 2022
Qian Zhang, Jiayu Zhang, Tong Lei, Zhanfeng Liang, Xue Dong, Liguang Sun, Yong Zhao
104732
,
1:300
dilution), L1CAM-PE (R&D,
clone
555, FAB5674P,
1:20
dilution), rabbit anti-DCLK1 (
Abcam
,
ab31704
,
1:15000
dilution), TCR-β-PE-Cy7 (
Biolegend
,
clone
H57-597,
109222
,
1:500
dilution),
mouse
CD1d loaded
(18)
Thrombin induces IL-8/CXCL8 expression by DCLK1-dependent RhoA and YAP activation in human lung epithelial cells
Journal of biomedical science
November 11, 2022
Yuliani FS, Chen JY, Cheng WH, Wen HC, Chen BC, Lin CH.
(Woburn, MA, USA) and
Invitrogen
(Waltham, MA, USA), respectively. The specific antibodies for DCLK1 (
ab31704
), YAP1 (
ab52771
), YAP phospho Ser127 (
ab76252
), thrombin (
ab92621
), and
E-cadherin
(
ab40772
) were procured
(19)
Microbial regulation of intestinal motility provides resistance against helminth infection
Mucosal Immunology
November 1, 2022
Mati Moyat, Luc Lebon, Olaf Perdijk, Lakshanie C. Wickramasinghe, Mario M. Zaiss, Ilaria Mosconi, Beatrice Volpe, Nadine Guenat, Kathleen Shah, Gillian Coakley,
et al.
mucosal integrity and no
artefacts
. Tuft cells
staining
was performed using anti-DCLK1 antibody (
Abcam
,
AB31704
) and anti-rabbit Alexa Fluor 488 (
Jackson ImmunoResearch
,
111-545-003
). Nuclei were then fluorescently
(20)
The proprotein convertase furin regulates the development of thymic epithelial cells to ensure central immune tolerance
iScience
October 21, 2022
Liang Z, Zhang Z, Zhang Q, Dong X, Yang X, Zhang J, Lei T, Creemers JWM, Zhang B, Zhao Y.
anti-K8 (DSHB, Troma-I),
chicken
anti-K5 (
Biolegend
,
905904
), and rabbit anti-DCAMKL1 (DCLK1) (
Abcam
,
ab31704
). Isolation of thymic
stromal cells
Mouse
thymi were isolated, cleaned of fat, and cut into pieces
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Anti-DCAMKL1 antibody from Abcam
Supplier Page
View Supplier Product Page
Pricing Info
Product Specs
Company Info
Citations
Reviews
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